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首页> 外文OA文献 >Evaluation of Three-Dimensional Chitosan-Agarose-Gelatin Cryogel Scaffold for the Repair of Subchondral Cartilage Defects: An In Vivo Study in a Rabbit Model
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Evaluation of Three-Dimensional Chitosan-Agarose-Gelatin Cryogel Scaffold for the Repair of Subchondral Cartilage Defects: An In Vivo Study in a Rabbit Model

机译：评价三维壳聚糖 - 琼脂糖 - 明胶Cryogel支架修复软骨下软骨缺损：兔模型的体内研究

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In this study, the potential of a chitosan-agarose-gelatin (CAG) cryogel scaffold for the repair of subchondral cartilage defects was explored in female New Zealand white rabbits. Custom-made CAG cryogel scaffold was implanted in a surgically created subchondral defect (diameter of 4 mm, depth of 4 mm) in knee joint of rabbit. The repair of the subchondral defect was evaluated at regular time interval by both macroscopic as well as microscopic examinations. The gross evaluation of the scaffold-implanted site showed integration of the scaffold with the surrounding tissue. Scanning electron microscopy and histological staining of the remnants of implanted cryogel scaffold showed infiltration of the host cells. The repair of the subchondral defect along with well-integrated regenerated cartilage was confirmed by the histology analysis of the joint. Results showed significant cartilage regeneration by the fourth week until eighth week after implantation. Immunohistochemical analysis confirmed that regenerated tissue is hyaline cartilage and absence of hypertrophy marker was reported. In addition, the CAG scaffolds did not elicit any adverse immunological rejection as shown by hematological analysis. Enzyme-linked immunosorbent assay did not show any statistically significant change in the concentration of tumor necrosis factor-alpha in the serum, and remained in a nontoxic range. Rabbits with a surgically created defect but no scaffold did not show any cartilage regeneration throughout the experiment of 8 weeks. These results demonstrate that CAG cryogel scaffolds promote repair of an osteochondral defect at a load-bearing site in rabbits.
机译：在这项研究中，探索了壳聚糖-琼脂糖-明胶（CAG）冷冻凝胶支架修复软骨下软骨缺损的雌性新西兰白兔的潜力。将定制的CAG冷冻凝胶支架植入手术产生的兔膝关节软骨下软骨缺损（直径4 mm，深度4 mm）中。通过肉眼和显微镜检查定期评估软骨下缺损的修复情况。对支架植入部位的总体评估显示支架与周围组织的整合。植入的冷冻凝胶支架残余物的扫描电子显微镜和组织学染色显示宿主细胞浸润。通过关节的组织学分析证实了软骨下缺损的修复以及良好整合的再生软骨。结果显示，在植入后第四周至第八周，软骨的再生能力显着。免疫组织化学分析证实，再生组织是透明软骨，并且没有肥大标志物的报道。此外，如血液学分析所示，CAG支架未引起任何不利的免疫排斥反应。酶联免疫吸附测定未显示血清中肿瘤坏死因子-α浓度的任何统计学显着变化，并保持在无毒范围内。在整个8周的实验中，具有手术造成的缺损但无支架的兔子没有表现出任何软骨再生。这些结果表明，CAG冷冻凝胶支架促进了兔子的承重部位软骨软骨缺损的修复。

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